WebMay 3, 2024 · HEK-293 cell line was cultured in Dulbecco’s modified Eagle’s medium (DMEM) medium (Thermo Fisher, Illkirch, France) containing 10% of heat-inactivated fetal calf serum (FCS) (Gibco Illkirch, France), 2 mM glutamine, and 1 mg/ml glucose. Stably transfected HEK-293 cells expressing membrane-bound IL-15Rα (IL-15Rα-HEK-293 … Web(Fisher, Illkirch, France) and sodium acetate (Sigma-Aldrich), at pH 4.5. They were then reacted with b-OEG-ONH 2 5 (3.4 mM; synthesized as described on page S3) in the presence of aniline (100 mM; Sigma-Aldrich) for 48 h at 37°C. Hydrazone ligation was performed as previously described.3 Briefly, HS suspended in phosphate-buffered saline …
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WebNot a Fisher Scientific Supplier? Learn more about becoming an eMarketplace Supplier for the U.S. Federal Government. Show me WebHEK-293 cell line was cultured in Dulbecco's modified Eagle's medium (DMEM) medium (Thermo Fisher, Illkirch, France) containing 10% of heat-inactivated fetal calf serum (FCS) (Gibco Illkirch ... WebAlveolar epithelial cells (AECs) play an important role in the fibrotic process as they undergo sustained endoplasmic reticulum (ER) stress, and may acquire a mesenchymal phenotype through epithelial-to-mesenchymal transition (EMT), two phenomena that could be induced by localized alveolar hypoxia. phoenix zoo discounts tickets