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Hepg2 dual

Web9 mar 2024 · Luciferase assays were performed 48 h after transfection using the Dual-Luciferase Reporter Assay System (Promega). ... HCCLM3 and HepG2 cells were transfected with si-HIF1A, ... WebInvivoGen provides HepG2 cell lines engineered to express the secreted embryonic alkaline phosphatase (SEAP) and Lucia luciferase reporter proteins for assessing the induction of …

Immunotoxin targeting glypican-3 regresses liver cancer via dual ...

Web11 apr 2024 · Human HCC cell lines MHCC-97H, HepG2, and HEK293T were obtained from the American Type Culture Collection. ... In this study, we identified this dual role of miR-3180 for the first time. First, miR-3180 expression correlated negatively with CD36 and SCD1 expression in patients with HCC. Web26 mag 2014 · The objective of this study is to investigate the contributing effect of the nuclear transcription factor-erythroid 2-related factor 2 (Nrf2)-mediated signaling pathway on the indirect antioxidant capacity of caffeic acid phenethyl ester (CAPE) against oxidative stress in HepG2 cells. The result of an antioxidant response element (ARE)-luciferase … diversity terminology uk https://thepowerof3enterprises.com

Synthesis, computational docking and biological evaluation of ... - PubMed

WebIn the present study, it was shown that IL-18 and its receptors (IL-18R) were constitutively expressed in hepatoma cell lines HepG2 and HepG2.2.15 as well as normal liver cell line … WebA dual near-infrared pH fluorescent probe has been designed, synthesized and applied to HepG2 cells, with a pKa value of 5.14 under acidic conditions and 11.31 under basic conditions, which is valuable for studying acidic organelles in living cells and pH changes in chemical systems. Web1 giu 2024 · These dual-functional immune-reagents have great potential in biosensors developing for diagnosis application. Abstract. ... Liver cancer cell lines, such as HepG2, Huh-7, Hep3B, SMMC-7721, SK-Hep-1cells were incubated with nanobody individually in PBS (2% BSA) buffer for 30 min with gentle shaking. After washing with PBS, ... diversity terms canada

Generation of dual functional nanobody-nanoluciferase fusion and …

Category:Establishment of a DGKθ Endogenous Promoter Luciferase Reporter HepG2 ...

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Hepg2 dual

Redox‐Responsive Dual Drug Delivery Nanosystem Suppresses …

Web1 giu 2015 · Overall, we have demonstrated that HepG2 cells acutely deprived of glucose are a suitable model for the identification of mitochondrial toxicity when used alongside … WebIntroduction. Cisplatin, cis-diamminedichloroplatinum (II) (CDDP), is a chemotherapy drug used in the treatment of solid tumors [1, 2].It exerts biological activity when it enters the cell and subsequently binds to DNA [] but cisplatin shows significant dose-related side effects such as nephrotoxicity, neurotoxicity, nausea and vomiting [].In order to maintain the …

Hepg2 dual

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Webline, HepG2-Dual™ cells did not respond to VACV-70/LyoVec™ stimulation. A moderate IRF response is detected in HepG2-Dual™ cells upon incubation with STING agonists …

Web7 apr 2024 · These results suggest that the dual-targeting PEAL-LA/VEGFab NPs could deliver miR-99a into HepG2 cells most effectively, which results in the inhibition of the proliferation of tumor cells and... Web15 feb 2024 · Monitoring the fluctuation of H 2 S in insulin-resistant HepG2 cells and diabetic mice with a dual-locked NIR fluorescent probe. Author links open overlay panel Zimeng …

Web1 nov 2024 · Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H 2 S/viscosity in the brain of drosophila Parkinson’s disease model. ... Web1 feb 2024 · A dual β-catenin knockdown HepG2 model allows study of independent structural and transcriptional activities of β-catenin. • WT and mutated β-catenins play antagonistic functions in hepatoblastoma cells. • Fascin-1 is a target of mutated β-catenin in tumour hepatocytes. •

Web11 mar 2015 · In HepG2 model, HN3-PE38 alone was less effective. As a single agent, 0.6 mg kg −1 HN3-PE38 only delayed HepG2 tumour growth. This was probably due to the different cell context of HepG2 in ...

Web5 apr 2024 · In this study, the novel multifunctional liposomes (CAPS-CUR/GA&Gal-Lip) were prepared for co-delivery of curcumin (CUR) and capsaicin (CAPS), in which glycyrrhetinic acid (GA) and galactose (Gal) were chosen as targeting ligands to modify the liposomes (Lip) for dual-targeting liver cancer. diversity test freeWeb1 set 2024 · After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. ... which led to the upregulation of dual-specificity phosphatase-9 (DUSP9), a direct target of miR-212, ... diversity terms in the workplaceWebDDIT3 Directs a Dual Mechanism to Balance Glycolysis and Oxidative Phosphorylation during Glutamine Deprivation. Mingyue Li, ... HepG2 cells transfected with either Flag control or Flag-DDIT3 were added protease inhibitor cocktail 1:25 before cell lysis and immunoprecipitations performed using control IgG (IgG-R) or anti-COQ9. diversity texas a\u0026mWebOverall, these studies demonstrate the utility of HepG2 cells for the identification of mitochondrial toxins which act directly on the electron transport chain and that the dual … diversity terms worksheetWeb15 feb 2024 · A novel dual-locked NIR fluorescent probe ( DLSP) was designed and successfully synthesized. • DLSP could selectively visualize the biosynthesis of endogenous H 2 S production in insulin-resistant HepG2 cells. • DLSP could be used to monitor the fluctuation of H 2 S in diabetic mice. Keywords 1. Introduction crack zamzom wireless network toolWeb11 apr 2024 · Dual luciferase reporter gene assay showed that miR-146a-5p can directly target FLAP mRNA. Knockdown of miR-146a also resulted in increased FLAP expression of cancer cells. Additionally, miR-146a silencing or restoration of FLAP led to a reduction of HepG2 cell proliferation, cell cycle progression, migration, and invasion. diversity testWebThen, the 96-well plate was shaken on a swing bed for 10 min at 37°C. Finally, the absorbance of the solution in each well was measured with a microplate reader (TECAN NanoQuant plate Infinite 200 Pro) at 570 nm to evaluate the viability of the HepG2 cells. Ability of the in vitro Dual-Modal Imaging diversity texas a\\u0026m