WebHieff® qPCR SYBR Green Master Mix(No Rox)是2×实时定量PCR扩增的预混合溶液。Mix中含有热启动Hieff® DNA Polymerase、SYBR Green I、dNTPs、Mg2+。使用时,仅需在扩增体系中加入模板和引物即可进行实时荧光定量PCR,大大简化操作过程,降低污染几率。 本品采用的DNA聚合酶配体可以随温度变化实时调节DNA聚合酶活性。 Web2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly ...
Hieff® qPCR SYBR Green Master Mix 11201ES 主营 - GuideChem
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SYBR™ Green PCR Master Mix - Thermo Fisher Scientific
Web7 de mar. de 2024 · Total RNA was extracted with plant RNA kit (OMEGA Bio-Tek Inc, R6827, Norcross, GA, USA) following the instruction. cDNA was obtained from TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, AT311-03, Beijing, China) by random primer follow the manual instruction. qPCR was performed … WebVazyme Biotech Co p621 01 hieff qpcr sybr green master mix yeasen P621 01 Hieff Qpcr Sybr Green Master Mix Yeasen, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Web10 de abr. de 2024 · Then qRT-PCR was performed using a Hieff qPCR SYBR Green Master Mix kit (Yeasen, Shanghai, China) and a LightCycler 96 (Roche Applied Science, Indianapolis, IN, USA). The sequences of primers are listed in Table 1. The expression of target genes was normalized to GAPDH and expressed as fold-change using the 2 … can lay on hands be used on yourself