Hieff trans
WebAs a cationic liposome transfection reagent, Hieff Trans™ liposome nucleic acid transfection reagent has higher transformation efficiency and more convenient … Web21 de mai. de 2024 · Core plasmids were co-transfected with psPAX2 and pMD2.G into HEK−293T cells using Hieff Trans TM Liposomal Transfection Reagent (Yeasen, Shanghai, China). 48 h later, virus supernatant was ...
Hieff trans
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http://www.labbase.net/News/ShowNewsDetails-1-22-66A109AF7D8B18F3.html Web上海翊圣生物科技有限公司是一家集研发、生产、销售为一体的高新技术企业。翊圣生物专注于生命科学和生物技术领域，致力于为客户提供一系列高质量和有绝对价格竞争力的产品，包括分子生物学、免疫学、生物化学和细胞生物学相关的试剂、试剂盒，并提供一系列相关的技 …
Web8 de mar. de 2024 · Lentiviruses were produced by co-transfection of the expression vector of interest with the packaging plasmids psPAX2 and pMD2G (Addgene) into HEK-293 cells using Hieff Trans™ Liposomal Transfection Reagent (Cat#40802, Yeasen, China). Virus supernatant was collected after 48 h. Transfected MM cells were screened by puromycin. Web30 de set. de 2024 · The siRNAs (50–100 n m) were introduced into cells using Hieff Trans liposomal transfection reagent following the manufacturer’s protocol (Yeasen). Cells were harvested 48–72 h after transfection...
WebProduct Name: Hieff TransTM Liposomal Transfection Reagent 2. Recommended use of the chemical and restrictions on use Relevant identified uses: For research use only. Uses advised against: Not used for diagnostic procedures. 3. Details of the supplier of the safety data sheet Yeasen Biotechnology (Shanghai) Co., Ltd. Web7 de abr. de 2024 · 通过调整DNA/Hieff Trans?脂质体核酸转染试剂比例优化转染效率,保证细胞密度大于90%,DNA(μg): Hieff TransTM(μL)比值在1:0.5-1:5。 7) 本产品仅作科研用 …
Web4 de nov. de 2024 · The plasmids of pcDNA3.1-control, pcDNA3.1-NRP1(HeWu, shanghai, China), and pcDNA3.1-YTHDF1(GenePharma, Suzhou, China) were transiently transfected into cells using Hieff Trans™ Liposomal Transfection Reagent (YEASEN, Shanghai, China). Cells were harvested after transfecting 24 h to 28 h for the following experiments.
WebHieff Trans™ Liposomal Transfection Reagent. Hieff Trans ™ is supplied in sterile liquid form. Generally, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections; For more details, … how do i uninstall an app on my iphone 13WebHieff Trans®脂质体核酸转染试剂是一种多用途的脂质体转染试剂，适用于DNA、RNA和寡核苷酸的转染，对大多数真核细胞具有很高的转染效率。 其独特的配方使其可直接加入 … how do i uninstall an email accountWeb1 de set. de 2024 · Overexpression of KLF2 in vitro inhibited growth, migration and invasion of RCC cells. Analysis of clinical specimens revealed that there is a close correlation between KLF2 and GPX4 in ccRCC. how do i uninstall an app that is not listedWebHieff TransTM siRNA/miRNA In Vitro Transfection Reagent is a non-liposomal PEI cationic, amphiphilic transfection reagent developed for transfection of siRNA into mammalian … how do i uninstall an app on windows 10WebHieff Trans™ PEI Transfection Reagent-GMP was used to transfect the suspension cell HEK293F cell line and adherent cell HEK293T cell line in a 6-well format. AAV-GFP … how much oil flows through keystone pipelineWeb3 de jan. de 2024 · Cells were plated in antibiotic-free DMEM (high glucose) supplemented with 10% FBS 16 h before DNA transfection. Then, 0.5 μg DNA with 1.5 μL of Hieff Trans TM liposomal transfection reagent were typically used for each well of a 4-chamber glass-bottom dish in accordance with the manufacturer’s protocol. Animal studies how do i uninstall and reinstall edgeWebHieff Trans™ in vitro siRNA/miRNA Transfection Reagent is a PEI transfection reagent developed for the transfection of siRNA into mammalian cells. Suitable for transfection of … how do i uninstall and reinstall bingo blitz