Irf reporter assay

Author: rxon

August undefined, 2024

WebHCT116-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1 binding sites. HCT116-Dual™ cells also … WebDescription. THP1-Dual™ cells feature the Lucia gene, a secreted luciferase reporter gene, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated …

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WebMar 10, 2024 · We performed ChIP assay to detect binding of IRF-3 ... Luciferase reporter assay. 293T cells were seeded in 24-well plates at 1 × 10 5 cells per well in DMEM … WebSep 15, 2005 · We developed a reporter assay system employing a self-inactivating retrovirus and analyzed the cystatin C and cathepsin C promoters. We found that a unique cis element mediates IRF-8-induced activation of both promoters. characteristics of a television drama

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WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … WebThe Interferon Regulatory Factor (IRF) reporter (Luc)-THP-1 cell line is designed to study the activation and signaling of Cytosolic DNA Sensors (CDS) in the human monocytic cell line … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … WebRather, PLpro interacts with IRF-3 and inhibits the phosphorylation and nuclear translocation of IRF-3, thereby disrupting the activation of type I IFN responses through either Toll-like receptor 3 or retinoic acid-inducible gene I/melanoma differentiation-associated gene 5 pathways. ... activation inhibit and luciferase reporter assay: 1, 2, 3 ... characteristics of a theory y manager

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IRF Reporter (Luc) THP-1 Cell line Catalog #: 79858 - BPS …

WebSep 22, 2024 · To perform the reporter assay, you clone the regulatory region of your gene-of-interest (X) upstream of the luciferase gene in one of these expression vectors, … WebReporter Gene Assays Reporter gene assays are invaluable for studying the regulation of gene expression by cis- or trans-acting factors, or for measuring activation of cell … harper baptist churchWebProduct overview. IRF3 Transcription Factor Assay Kit (Colorimetric) (ab207210) is a high throughput assay to quantify IRF3 activation in nuclear extracts. This assay combines a … harper band ring in gold

"WebMar 29, 2024 · Dual-luciferase reporter assay Artificially synthesized SOX4 promoter sequence fragments were inserted into the pMIR-reporter (Promega) after enzyme digestion. Next, the correctly sequenced luciferase reporter plasmids were respectively cotransfected with LV-NC, LV-LIN00470, LV-EGR2, si-NC, and si-LINC00470 into U87 cells. " - Irf reporter assay

Irf reporter assay

IRF Family of Transcription Factors as Regulators of Host Defense

WebJun 10, 2016 · Enzyme-linked Immunosorbent Assay (ELISA) Three days post-transfection afterstimulation 12hours, humanIL-8 immunoassay (eBioscience) performedaccording manufacturer’sinstructions. RAW264.7 stable knockdown cell lines were stimulated 16hours murineIL-6 immunoassay (R&D Systems, Inc.) performedaccording … WebBoth reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent. Figures Back to the top Specifications Antibiotic resistance: Blasticidin and Zeocin® Guaranteed mycoplasma-free Quality Control:

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WebFeb 5, 2024 · ACE2-(TMPRSS2) expressing cells HEK-Blue™ hACE2 Cells A549-hACE2 Cells A549-hACE2-TMPRSS2 Cells이 세포들은 세포 표면에서 높은 수준의 host receptor ACE2를 단독 혹은 host protease TMPRSS2와 결합하여 발현합니다. 따라서 이러한 세포는 SARS-CoV-2 Spike pseudotyped lentiviral particle에 의한 감염이 가능하며, 특히 A549 세포에 … WebTumor heterogeneity dominates tumor biological behavior and shapes the tumor microenvironment. However, the mechanisms of tumor genetic features modul…

WebInterferon regulatory factors (IRFs) constitute a family of transcription factors that commonly possess a novel helix-turn-helix DNA-binding motif. Following the initial … WebApr 11, 2016 · Established the manufacturing, chemistry, biochemistry and TC laboratories. Validated cell culture methods, reporter assays and lysate experiments on SF9, 3T3 and …

WebTranscription factors of the interferon regulatory factor (IRF) family have been identified as critical mediators of early inflammatory gene transcription in infected cells. We recently determined that, besides IRF-3 and IRF-7, IRF-5 serves as a direct transducer of virus-mediated signaling. WebIRFs are required to submit quality data for the IRF Quality Reporting Program beginning on 10/01/2012. During the first year of the IRF Quality Reporting Program, IRFs are required …

WebCustom DNA Oligos, RNAi & Assays. R&D Systems™ Luminex™ Assays Tool; Centrifuges & Microcentrifuges. Centrifuges & Microcentrifuges. Benchtop Centrifuges; Centrifugal Filter …

WebNOTE: The IRF Provider Preview Reports will not be in your facility’s Validation Report (VR) folder. 5. Selecting the desired IRF Provider Preview report link from the list of reports … characteristics of a thiefWebOct 30, 2015 · For each inpatient on the list, the IRF must include the payer the IRF can bill, or has billed, for treatment and services furnished to the inpatient. If an inpatient on the … harper baptist church glasgowWebOct 14, 2024 · The anti-inflammatory activities of water extract and individual compounds were screened by NF-κB and interferon regulatory factor (IRF) reporter assays in THP-1 … characteristics of athletes footWebAug 1, 2009 · IRF4 directly binds the Il10 gene as evidenced by chromatin immunoprecipitation assay, and regulates Il10 control elements in a reporter assay. IRF4 … characteristics of athens and rome governmentWebCell-Based Assays Luciferase Reporter Stable Cell... harper bariatric medicine instituteWebApr 12, 2024 · The global Reporter Gene Assay market size is projected to grow from USUSD 1344.7 million in 2024 to USUSD 2010.3 million in 2029; it is expected to grow at a CAGR of 5.9Percent from 2024 to 2029 ... harper barbecue pitsWebPNT2258 is a BCL-2 -targeting DNAi molecule which contains a single stranded sequence of 24 bases of unmodified phosphodiester DNA (known as PNT100) with a specific … characteristics of a tight labour market