Nephrin antibody
WebNephrin not only functions as the core component of the extracellular SD filtration network, ... 1193, and 1217 is the most widely characterized, potentially due to the commercial availability of phospho-specific antibodies for these sites . Phosphorylation at these sites results in the recruitment of Nck, PLC-γ1, ... WebNov 14, 2024 · Phosphorylation of nephrin can be induced using FKBP-FRB heterodimers. Nephrin is an essential structural component of the kidney glomerulus, a specialized structure for ultrafiltration of plasma, consisting of eight extracellular immunoglobulin G (IgG) repeats, a transmembrane domain, and an unstructured tail of ∼160 amino acids …
Nephrin antibody
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WebNephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin.The immunogen is located within the last 50 amino acids of Nephrin. 纯化方式 Purified by antigen-affinity chromatography 偶联 Unconjugated. … WebMar 2, 2024 · The discovery of anti-nephrin antibody “dusting” in these biopsy specimens is accompanied by the presence of high levels of anti-nephrin antibody in the serum. It …
WebThis case strengthens evidence that anti-nephrin antibodies cause disruption of the slit pore diaphragm which appears to be readily responsive to immune therapy. Anti-nephrin mediated podocytopathy may present with a spectrum of glomerular histopathology, which on the background of other susceptibility factors, can lead to more severe presentations … WebJul 22, 2024 · nephrin Antibody (B-12): sc-377246 nephrin Antibody (B-12) is a mouse monoclonal IgG 2a κ, cited in 25 publications, provided at 200 µg/ml specific for an...
WebNov 3, 2024 · 17, 18 Anti-nephrin antibodies were detected in 29% of MCD patients in initial disease onset and then became undetectable in remission. 17 In these patients, delicate punctate IgG deposits were ... WebNephrin antibody was raised in guinea pig using a synthetic peptide corresponding to residues 1243-1256 of the intracellular domain of nephrin as the immunogen. Validations. None provided. Applications. Immunohistochemistry (IHC) Immunohistochemistry-paraffin (IHC-P) Western Blotting (WB) Reactivity. Homo sapiens (Human) Mus musculus …
WebAug 7, 2024 · A large part of why nephrin phosphorylation is well characterized compared with other podocyte proteins is because of the existence of several reliable phospho-nephrin antibodies. However, phospho-specific antibodies can be difficult to generate with the specificity needed to reliably detect and quantify the phosphorylation event of interest.
WebJun 20, 2024 · Antibodies and immunoblotting. Purified rabbit polyclonal antibodies against nephrin, p-nephrin antibodies have been described previously [14–17].β-actin and synaptopodin antibodies were obtained from Sigma-Aldrich.Proteins were extracted from plasma membranes in RIPA buffer (PBS containing 0.1% SDS, 1% Nonidet P-40, 0.5% … downtown kc nail salonWebNephrin is closely associated with the slit diaphragm of the glomerular podocyte, and has recently received attention as a potential therapeutic target. The function of nephrin, ... clean freak ending field trip zWebNephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the … clean freak floor bufferWebNephrin Antibody detects endogenous levels of total Nephrin. RRID: AB_2844314 Cite Format: Affinity Biosciences Cat# AF7951, RRID:AB_2844314. Conjugate: Unconjugated. Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Storage: ... downtown kearneyWebNephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # … downtown kc ballparkWebPurified by Protein A. Storage Buffer. Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Condition. Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Target. Gene ID. 4868. downtown kearney storesWebNephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. clean freak dry shampoo medium